Isaac Adeyemi Adeleye
University of Lagos, Nigeria
Title: Whole genome ampliï¬ cation of torque teno virus from Nigeria HIV patients and blood donors
Biography
Biography: Isaac Adeyemi Adeleye
Abstract
Torque teno virus (TTV) is a single stranded DNA virus suspected to be responsible for hepatitis symptoms in humans. There is no published data on the genomic characterization of TTV in Nigeria. To this end, 260 plasma samples, 130 each from HIV positive patients and healthy voluntary donors were tested for TTV DNA by PCR and three near complete genome of TTV were isolated and sequenced. Using appropriate primers, genome amplification with the expected product size of 3652 bp was achieved. In-silico analysis was done to characterize this virus. The TTV Nigerian isolate FL100 yielded a product size of 3623 bp and shares the highest sequence similarity of 93%, E-value 0.0 over a 99% query cover, with a US isolate KT163879 (3748 bp). Both isolates clustered with other isolates from group 1 such as AB017610 (TTV prototype reference genome from US) AF298585, a Polish isolate. Isolate FL100 has between 63%-65% identity with other isolates in genogroup 1. It shares between 52%-59% identity with isolates from other phylogenetic groups. It has a 45% and 46% identity with TTMV and TTMDV respectively. Its alignment identity with other Nigerian isolates was 56% and 57% for FL08 and BD 67 respectively. FL100 has a pairwise distance value of 0.07 with KT163879 and 0.51 with group 1 reference isolate TA278 (AB017610) over the entire sequence. This confirms that the Nigeria isolate FL100 belongs to phylogenetic group one.